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Part:BBa_K1773013:Design

Designed by: Sarunas Tumas   Group: iGEM15_Vilnius-Lithuania   (2015-08-23)


Lux Right promoter with strong RBS sequence and Cas3 protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2725
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1446
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2409
    Illegal AgeI site found at 2782
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

Cas3 protein was cloned from Aggregatibacter actinomycetemcomitans D-7S strand bacterium.

References